Probability of a transmembrane domain

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Your PI has asked you to begin a project that she hopes will begin to define how a novel receptor is activated. You analyze the protein sequence using pBLAST and notice that different parts of the novel sequence align with some transmembrane proteins. Additional in silico analysis reveals that one of these regions has a high probability of being a "transmembrane domain".

A. Design an experiment to test whether this novel protein is a membrane protein and include relevant controls.

B. What experimental strategy could be used to test whether the region identified in your in silico analysis really is a transmembrane domain?

Reference no: EM133228557

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