Primary amino acid sequence of the products

Assignment Help Biology
Reference no: EM132016838

The tetrapeptide Trp-Arg-Phe-Glu at pH 7 was digested with trypsin and then fully deprotonated by adjusting to pH=14. The digestion products were then applied to a strong anion exchange column (positively charged). The peptides were then eluted from the column using a pH gradient from 14 to 0.5. Write the primary amino acid sequence of the products (full structures not required) in the order they would elute from the column. Indicate the pH value at which each product elutes from the column. Assume pKa's for any newly generated a-amino and a-carboxyl groups are 9.5 and 2.1, respectively.

Reference no: EM132016838

Questions Cloud

Calculate the yield to maturity of the bond using calculator : Calculate the yield to maturity of the bond using a calculator. Calculate the duration of the bond two ways: Using a calculator and using a spreadsheet.
Illustrate the advantages of your proposed solution : Using feedback from your professor and classmates, revise Parts 1 and 2, and add Part 3. Plan to include visuals to illustrate the advantages of your proposed.
Atp synthase two functional domains : Describe these two domains in term of its structures and functions? Why are they necessary for ATP synthesis? What would happen if one of the domains.
Capm and required return-capm and portfolio return : The risk-free rate of interest is 5.5%. By how much does Beale's required return exceed Foley's required return?
Primary amino acid sequence of the products : Write the primary amino acid sequence of the products (full structures not required) in the order they would elute from the column.
Is organic food healthier than conventional food : What are the advantages and disadvantages of consuming organic food? Is organic food healthier than conventional food? Why or why not?
Indicate clearly the volume of naoh needed : Indicate clearly the volume of NaOH needed to reach each relevant pKa value, the isoelectric point (pI) and equivalence point(s).
Discuss the work meaning from a political perspective : Choosing one of the films from the list below, discuss your understanding of the work's meaning from a political perspective, and evaluate the formal choices.
Ph and concentration of the buffer solution : Calculate the pH and concentration of the buffer solution. The relevant pKa values are 2.2 and 9.5

Reviews

Write a Review

Biology Questions & Answers

  Immune response to infectious disease

It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  Plastic as a soil stabilizer

Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.

  Principles of microbiology

This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.

  List the biologic functions

Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?

  Biologic function relationships

Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.

  Case study on patient in the haematology laboratory

Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.

  Use of pcr and genetic approaches in biotechnology

The use of PCR and genetic approaches in biotechnology

  Describe the role of this enzyme in honey

Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.

  Genetic problems

What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?

  Prepare an essay on nosocomial infection

Prepare an essay on nosocomial infection.

  Monitoring and recording the blood pressure

To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd