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The tetrapeptide Trp-Arg-Phe-Glu at pH 7 was digested with trypsin and then fully deprotonated by adjusting to pH=14. The digestion products were then applied to a strong anion exchange column (positively charged). The peptides were then eluted from the column using a pH gradient from 14 to 0.5. Write the primary amino acid sequence of the products (full structures not required) in the order they would elute from the column. Indicate the pH value at which each product elutes from the column. Assume pKa's for any newly generated a-amino and a-carboxyl groups are 9.5 and 2.1, respectively.
Where can someone find out what a recommended serving size should be for ice cream or pasta?
In clown fish, the allele B for blue scales is dominant over the allele b for yellow scales. On another chromosome, the allele N for long tail fin is dominant over its allele n for short tail fin. Give the most probable genotypes for the parent cr..
Diseases caused by eukaryotic parasites are much harder to treat than bacterial infections why?
Imagine that I start off with 100 molecules of oxaloacetate in a mitochondria. After running the TCA to generate 20 molecules of CO2
Enzymes secreted by the pancreas are involved in all of the following digestive reactions with one exception. What is the exception?
What are bypass polymerases? How do they differ from the replicative polymerases? How do their special features facilitate their role in DNA repair?
The final concentration of a compound A is 10 mg/mL after performing three 1:2 serial dilutions. What was the starting concentration?
Since L-glucose cannot be metabolized, it has no caloric value. Why is L-glucose not the perfect diet aid? An uncatalyzed reaction has a rate of 4.2 x 10-7 sec-1. When an enzyme is added the rate is 3.2 x 104 sec-1. Calculate the rate enhancement c..
1. Explain the function of all molecules involved in DNA replication. 2. Explain the details of DNA polymerase structure and associated functions. 3. What are the different types of DNA polymerase in prokaryotes and eukaryotes and what are their func..
Bacteria that live in extremely hot water are called thermophiles. If you looked at a particular enzyme in such bacteria, do you think that it might have more or fewer cysteine amino acids than bacteria living at moderate temperatures?
A teacher makes the analogy that stem cell research did for medicine what the Internet did for communication. What is meant by this analogy?
A method for separating polypeptides makes use of their differentail solubilities. The solubility of huge polypeptides in water depends upon the relative polarity of their R groups.
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