Reference no: EM132537654

1) You are studying the metabolic capabilities of a species of bacteria by growing cultures on different carbon sources and comparing their mRNA expression profiles using microarrays analysis. You grow one culture on the single carbon compound methanol , and another culture ( same species) on the four carbon compound succinate. You extract the mRNA from each culture and use microarray analysis to obtain the following expression ratios for three sample genes comparing growth on methanol to growth on succinate (i.e. ratio is methanol : succinate). Gene A:0.998 ; Gene B:0.0037; Gene C:32.7 for each gene, propose a general role based on this expression data that demonstrate your understanding of gene expression and microarrays.

Gene A:

Gene B:

Gene C:

2) an extensive and comprehensive research projects is conducted in which a single species of bacteria is grown in two different steady state chemostats under completely different growth conditions with the goal of understanding how the species differs under these two forms of growth conditions. The two cultures are grown, and then mRNA, proteins, and DNA are all extracted and quantified as follows:

1) microarray analysis

2)proteomics-proteins present quantified via GC-mass spectrometry ( gas chromatography -mass spectrometry a technique that allows quantification of all the proteins present in a sample)
3)complete sequencing of all the mRNA present

4) complete sequencing of all the DNA present
for each of these, briefly explain what each approach would yield , i.e., what would it tell us about the species of bacteria grown under the two different conditions? please explain briefly but clearly for each of the four approaches.

a) microarrays analysis

b) proteomics

c)mRNA sequencing
d)DNA sequencing

of these four approaches, which two do you think would yield the closest set of information? explain?