Presence of nontransformed bacteria

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Question

What might explain the presence of nontransformed bacteria growing on the LB/amp plate?

The ampicillin in the medium was not working.The ampicillin in the medium was not working.

The bacteria were already naturally resistant to ampicillin.The bacteria were already naturally resistant to ampicillin.

Too much heat was applied during the heat shock phase.Too much heat was applied during the heat shock phase.

The plasmid gave the bacteria the ability to express the fluorescing protein.The plasmid gave the bacteria the ability to express the fluorescing protein.

Reference no: EM133313716

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