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Assume you perform a colorimetric enzyme assay to determine the activity of invertase in a bioreactor (total volume = 1L) used to produce inverted sugar. You prepare your standard curve by mixing known monosaccharide dilutions (3mL) with the DNS reagent (2mL). The standard curve follows the following linear function: Absorbance (540nm) = 1.82 X monosaccharride concentration (M)
You mix 1.5 mL of the enzyme solution from the bioreactor with 1.5 mL of substrate solution and let them react for exactly 10min, after which you stop the reaction by adding 2mL of DNS reagent. You then add 20mL of distilled water and read the absorbance value at 540nm.
You obtain an absorbance value of 0.395.
Determine the reaction rate (mol monosaccharide formed / min) in the bioreactor.
In dogs, colored fur is dominant to un-colored fur. Black fur color is dominant to brown fur color. The E gene is epistatic to the B gene.
Explain how cell division and the sexual reproduction help in genetic variability of organism. How do both the meiosis and sexual reproduction (fertilization) generate offspring which differ genetically from parents?
Down syndrome is the result of an extra 21st chromosome in humans. In the process of meiosis for the mother, give all possible stages where it could lead to an offspring with Down syndrome,
You have at your disposal a variety of supplies including antacid tablets, water and film canisters.When you mix these effervescing tablets with water,
The restriction enzyme Scs1 cuts at a restriction site that is found only very rarely in dog genomes. One dog, Jimmy, has two restriction sites for this restriction enzyme in his genome. Another dog, Billy, has three restriction sites for this enz..
The Twenty mL of water is added to the Erlenmeyer flask is to previously boiled, deionized water. Since water does absorb CO2 from atmosphere and since CO2 dissolved in water causes it to be slightly acidic,
Describe why you can predict that meiosis gives rise to genetic differences between parent's cells and daughter cells in fewer cell divisions than mitosis does.
Which of following statements is NOT true about the chemical bonds? Both kinds of the chemical bonds involve electrons in the outer shell. Covalent bonds share the electrons between two atoms.
A marine biologist has dredge up some unknown animal from the seafloor. Describe some of the characteristics she must look at to determine the animal phylum.
What are the Gram reactions of Clostridium and Bacillus. Unfortunately I have to submit this question which is part of my interactive computer assignment shortly that's why such a short notice. Today is a cut off day and I want to submit my assignmen..
How does your individual TRC and those of the average of class compare with the average TRC of the general population as reported by Holt (1968) as 145 for male and 126 for female.
In the fruit fly, the wild type purple eye color is produced through a combination of brown xanthommatin and bright-red drosopterin pigments.
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