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Imagine that you have engineered a set of genes, each encoding a protein with a pair of conflicting signal sequences that specify different compartments. If the genes were expressed in a cell, predict which signal would win out for the following combinations. Explain your reasoning.
A) Signals for import into nucleus and import into ERB) Signals for import into peroxisomes and import into ERC) Signals for import into mitochondria and retention in ERD) Signals for import into nucleus and export from nucleus
Use the cleland representation create the reaction progress of thrombin, a serene protease using a ping pong mechanism on the hydrolysis of the substrate Ala-Arg-Gly-Ala
Rotonone inhibits Complex I Cyanide inhibits Complex IV Oligomycin inhibits ATP synthase You incubate isolated intact mitochondria in a buffered solution containing NADH, ADP plus Pi.
In SDS-Page, proteins are separated by length. If the same length proteins can fold into loose (bigger) or condensed (smaller) shapes and can even complex with other proteins. How can we make an antibody to a specific protein?
Explain the lochia in a C-sec as compared to a vag delivery. Name and define the three words that are commonly used to describe lochia in its various forms.
In humans the gene for right handedness is dominant to the gene for lefthandedness. A man and a women both heterzygous for this trait to marry. What percent of thier children.
Assume your professor handed you a test tube with 3.0 ml of an E.coli culture in it and told you to make a 10^(-3) dilution of the entire culture. Describe how you would do it.
Who wrote the essay that inspired both Darwin and Wallace to develop the theory of natural selection? What did the essay say?
Assume you remove your test cultures from the incubator and notice that one of them- a know fermenter- has a gas bubble in the Durham tube.
Hematopoietic stem cells cultured in the presence of which receptor differentiate into T cells rather than B cells?
How does aggregation occur in sickle-cell anemia? Place the steps in the correct order. Note that deoxyhemoglobin is generally in the T state; oxyhemoglobin is in the R state.
explain some of the bioethical concerns related to screening for genetic diseases.
Preliminary trials suggest that the drug tamoxifen, antiestrogen commonly use to treat breast cancer, is useful in treating biopolar disorder. Tamoxifen is an inhibitor of Protein Kinase C.
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