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DNA Polymerase can distinguish between dNTPs and rNTPs because of discriminator amino acids in the enzyme's nucleotide-binding pocket. These amino acids occupy the space where the 2'OH group of an incoming rNTP would need to reside in order to properly position the substrates for catalysis. These discriminator amino acids usually have large R groups, which sterically exclude the ribose 2'OH. If you experimentally mutate/change the discriminator amino acids to glycines, predict the effect that this change might have on DNA polymerase.
You need to develope an experiment to test your product. you have thirty volunteers with colds to help you. Include the following parts. Definition of the problem, Hypothesis, Variable, control procedure and experimental procedure.
What is the current status of the Age Discrimination in Employment Act? Is the legislation really necessary? Why or why not
Who discovered that traits are inherited following mathematical laws and Who discovered the structure of DNA
q. a haploid strain of aspergillus nidulans carried an auxotrophic met-8 mutation conferring a requirement for
The hypothesis that was being tested was about the anti-predation behaviour exhibited by the while-tailed deer and how the response is affected in the presence of the predator - Did the introduction develop the hypotheses logically
What aspects of social and work environment in a college cafeteria will be of particular interest to a sociologist since of his or her sociological imagination.
Why do you think a large part of the primary somatic sensory area of the cerebral cortex is devoted to the face and hands?
in 200 to 300 words the path food follows through digestive system and how digestion happens in each of following parts
describe the concept of semi-conservative replication and the experiment that showed this. Describe the experiment that was used to demonstrate that DNA was replicated bi-directionally.
Which is a competitor of acetylcholine? Malathion or EDTA? Malathion inhibits the enzyme acetylcholinesterase, which hydrolyzes acetylcholine. EDTA binds free calcium ions.
Haemophilus influenza requires hemin to synthesize cytochromes and NAD from other cells. For what does it use these two growth factors? What disease(s) does H. influenza cause?
How many cups of the coffee would you have to drink to reach the dose (10mM) needed to interfere along with the DNA replication checkpoint mechanism?
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