User Account

All Pages

Polymerase chain reaction followed by gel electrophoresis

Assignment Help Biology
Reference no: EM132621059

1. What fragment would remain if the DNA fragment 5'-TCCTGTACAACGACC-3' were cut using a restriction enzyme that has a four base pair palindromic recognition site? After the cut, two bases are left unpaired as sticy ends and a base apir at each end of the palindrome is left paired.

2. If the DNA fragment 5'-GCAAGCTTGCTGGGCCCAGAATTCCCGGA-3' was digested using five restriction enzymes as below separately and then a gel was prepared where each digest was in a separate well, what would the results from running the gel look like?

  • EcoRI 5'-GAATTC-3'
  • SmaI 5'-GGGCCC-3'
  • BamHI 5'-GGATCC-3'
  • AluI 5'-AGCT-3'
  • HindIII 5'-AAGCTT-3'

 

3. How would the procedure, analysis, and outcome differ from running a polymerase chain reaction followed by gel electrophoresis?

Reference no: EM132621059

Questions Cloud

Quantitative and qualitative research and data in business : Discuss the value and use of quantitative and qualitative research and data in business.
Identify the time intersection she will need to determine : What are at least two methods she can use to identify the time/space intersection she will need to determine a list of incident witnesses? Please explain your.
Gene on the homologous chromosome : Will this type of mutation affect the copy of this gene on the homologous chromosome?
What is the total contribution margin at break-even point : Victoria Company sells a single product. Without resorting to computations, what is the total contribution margin at the break-even point?
Polymerase chain reaction followed by gel electrophoresis : How would the procedure, analysis, and outcome differ from running a polymerase chain reaction followed by gel electrophoresis?
Identify the environmental and organizational pressures : Identify the environmental and organizational pressures currently driving organizational change in your field or industry.
Describe your conflict system : Describe your conflict system (avoidant, aggressive, or collaborative). Include brief examples or experiences that reinforce your personal and professional.
Compute the overall break-even point for the company in sale : Compute the overall break-even point for the company in sales pesos. A contribution format income statement for a recent month for the two games
How you can apply the knowledge to better your community : In this video describe the ways you can change your behaviors, and explain how you can apply this knowledge to better your community.

Reviews

Write a Review

Biology Questions & Answers

  Describe the chain of infection specific to disease

Identify an infectious disease that is considered emerging or re-emerging. Using credible sources, research the agent, host, transmission process and the environment associated with this disease.Describe the chain of infection specific to this diseas..

  Did the mutations result in a change in the final proteins

backgrounddna represents the architectural blueprint for all living systems encoding specific instructions in the

  What genotypic ratio is expected

when mendel crossed yellow-seeded and green seeded pea plants, all the offspring were yellow seeded. when he took these F1 yellow-seeded plants and crossed them to green-seeded plants, what genotypic ratio is expected?

  Ab blood and a man has type b blood

If a woman has type AB blood and a man has type B blood, could they produce a child with t ype A blood? Explain using a Punnet square.

  Describe the different cultural characteristics

Describe the different cultural characteristics identified when you use agar plates, agar slants, and nutrient broth.

  Perform a blast search

Perform a BLAST search and a result has an E value of about 1 x 10-4. What does this E value mean? What are some parameters on which an E value depends?

  Enzyme necessary for the synthesis of folic acid

Sulfanilamide was the first antibiotic. It acts as a competitive inhibitor of an enzyme necessary for the synthesis of folic acid. Folic acid is our vitamin B9.

  Example of benefit or harm to life that hinges on an enzyme

An example of benefit or harm to life that hinges on an enzyme. For example, some diseases are the result of an underproduction or overproduction of a vital enzyme. Also, some substances that are considered poisons or pollutants do their harm..

  Refresh your memory on earlier concepts

Now, we'll spend the next four weeks reviewing and doing activities that hopefully will refresh your memory on earlier concepts.

  What are the variables you will need to control

For the scientific method lab, you will design your own experiment and then run it!

  Part of the brain-medulla oblongata-pons and midbrain

Choose one part of the brain (medulla oblongata, pons, midbrain, thalamus, hypothalamus, epithalamus, cerebellum, or one of the cerebral lobes).

  Calculate the heterozygous phenotype

In particular African region, 67% if the female population and 78% of the male population are resistant to malaria and are actively involved in teaching African Tribal dance to the tourist.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

Submit Assignment

Academics

Major Subjects

Majors

Get In Touch

whatsapp: +1-415-670-9521

Phone: +1-415-670-9521

Email: [email protected]

TERMS & POLICIES

HELP & SUPPORT

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd