Reference no: EM133232514

The following is the DNA sequence of the wild type allele of Gene Z that you want to amplify using the polymerase chain reaction (PCR).

5' CTCGAGGTGAATATGAAAG--------------------CATTTGGCGCGTAATCGATA 3'

3' GAGCTCCACTTAT ACTTT C--------------------GTAAACCGCGCATTAGCTAT 5'

1. If you amplify a DNA sequence through PCR what are the reaction components that you would absolutely need? Briefly state the function of each of these components.

2. What set of primers would you use for PCR reaction in the wild type allele of Gene Z?

3. State 5 uses of polymerase chain reaction (PCR)