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1. The polylinker sequence for pQE30 is shown in the box below:
pQE30 polylinker sequence
121 131 141 151
5'ATGAGA GGATCGCATC ACCATCACCA TCACGGATCC GCATGCGAGC
161 171 181 191 201
TCGGTACCCC GGGTCGACCT GCAGCCAAGC TTAATTAGCT GAGCTTGGAC3'
On the polylinker sequence above, identify and clearly label the following features. For parts (ii) and (iii), refer to the genetic code (codon translation table) provided.
(i) The BamHI and HindIII restriction sites
(iI) The region of the DNA encoding the initiating methionine.
(iiI) The region encoding the hexahistidine tag.
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