Physical protein structure of rgfp

Reference no: EM1388626

Question: Chromatography have a limited protein binding capacity. During the Ni+2 Agarose Column lab, you noticed that the wash 2 (W2) fluoresced slightly. Based on the Western Blot antibody procedure, would you expect to see a band in the W2 lane? If sp wjay anpit be its molecular weight? What does this expected data day about the physical protein structure of rGFP?

Note: the Wash 2 was not overloaded in the Ni+2 agarose column. The protein used is rGFP, which contains His 6 tag on its
N-terminus and express epitope tag on its C-terminus. The MW of the enitre rGFP protein is 33 kD and His 6 tag and express epitope is 6 kD each. Not that the antibody binds at the express epitope tag and not the His 6 tag.

Reference no: EM1388626

Questions Cloud

Eparate instruments with lenders and borrowers : Financing created by an intermediary which involves separate instruments with lenders and borrowers is called illustrate what?

Create the diagram 0 dfd for new century : Create the diagram 0 DFD for New Century. Make sure to illustrate numbered processes for handling appointment processing, payment and insurance processing.

Type of research uses numeric measurement data : a) What type of research uses numeric measurement data? b) What type of research uses a research hypothesis? c) What type of research does not use statistical data analysis?

What is the acceleration of each of the hanging masses : A uniform disc of mass m and radius R is rigidly mounted coaxially with a lightweight disc of radius r = R/2, to a horizontal shaft, on which combination is free to rotate frictionless. Light strings are wound in opposite directions about the cyli..

Physical protein structure of rgfp : Chromatography have a limited protein binding capacity. During the Ni+2 Agarose Column lab, you noticed that wash 2 (W2) fluoresced slightly.

Test and scale examples from discipline : Describe the test and scale examples from your discipline, including the populations, the reliability and validity of each test, and whether the test and scale could be applied to another population.

Create a threat model for your own blog setup : Create a threat model for your own blog setup. Additionally, explain the purpose of building a threat model, and how it applies to this case.

Explanation for parts of the neuron : Explain the following parts of the neuron and give the function of each part, Describe what is happening when you stare at the image, than transfer your gaze to a blank sheet of paper.

Public heatlh epidemiology : In epidemiology, demographic data such as age, sex, race, ethnicity, social class, occupation, marital status, etc. contribute to variations in health status, health-related behavior, and use of health care services.

User Account

All Pages