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Perfect image of themolecule with the ideal lens

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• A perfect negative index lens is used with a single molecule.How long time will it take to form a perfect image of themolecule with the ideal lens, and why?

• In STORM and PALM microscopy the spatial position offluorescent molecules is accurately determined in thetransverse plane (xy). Explain how their location may bedetermined in depth (z)?

• An evanescent wave is generated at the surface of a prism bytotal-internal reflection of a light beam. In which direction doesthe evanescent wave propagate (along, perpendicular or at anangle with respect to the surface)? Does it carry any energy?

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