Pcr to sequence a particular gene

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Reference no: EM1382035

Question: Assume you wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volume. How would you dilute your primer, which is currently at 1mg/ml to provide 3.2pmole in 2 micro liters if the molecular weight of your primer is 13,425?

Reference no: EM1382035

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