Pcr derived dna fragment, The PCR-derived DNA fragment used

Pcr derived dna fragment

Assignment Help Biology

Reference no: EM1390204

The PCR-derived DNA fragment used an insert in pUC19 was amplified by 25 cycles.
The amplification mixture contained, among other components, 1 ng lambda DNA in 50 uL.
If you assume that the PCR amplification process and restriction digestion process are 100% efficient, how many pmol of product (1110 bp fragment) were produced?
How mnay ug of DNA (1110 bp product) were present after amplification.
The 1110 fragment was digested with BamHI and HindIII to form the 784 bp product. How many ug of DNA (the 784 bp product) were formed? Average MW of 1 bp= 660 g/mol

Reference no: EM1390204

Questions Cloud

Find the product of the second polynomial : Find the product of all the three zero of the second polynomial

Process and the number of defectives : Fifty samples of size 600 were drawn from a manufacturing process and the number of defectives in each sample was counted.

Instead of a separate performance appraisal system : Instead of a separate performance appraisal system, Nucor judges performance based on bottom line of quality, productivity, and profitability.

Distributions compare for the various sample sizes : f x has a normal distribution with mean µ=15 and standard deviation σ= 3, describe the distribution of x values for sample size n, where n = 4, n = 16, and n = 100. How do the x distributions compare for the various sample sizes?

Pcr derived dna fragment : The PCR-derived DNA fragment used an insert in pUC19 was amplified by thenty-five cycles. Suppose that the PCR amplification process and restriction digestion process are 100% efficient.

Illustrate out the term control chart : Illustrate out the term control chart? Critically discuss the grand mean, the UCL and the LCL of a control chart for the mean?

Project management is a strategic necessity for the success : Project Management is a strategic necessity for the success of any business. Based on the reading in the chapters what do you think are the 5 most critical points in delivering successful Project Management

Information about control charts : The following data shows the packages gathered for delivery for ten days and the proportion of packages received not delivered to the customers at expected time.

Microsoft''s and apple''s organizational cultures : Are there differences between Microsoft's and Apple's organizational Cultures? Why did Apple's organizational culture disrupt the company?

User Account

All Pages