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You're trying to PCR amplify a gene, but your gel shows that there are two bands instead of only one like you expected. You know for certain that there no homologous genes in the genome from which you're amplifying, so you assume that the issue must be coming from your primers or PCR conditions. Briefly describe 2 different strategies for solving the problem and how they work.
5. You have sequenced a human protein gene from genomic DNA. You would like to use E. coli as mechanism to express this protein. Identify and briefly describe two modifications you might have to make to make to your sequence in order to have successful expression.
6. Restriction fragment length polymorphism (RFLP) analysis is commonly used for tracing the relatedness of pathogenic microorganisms in food-related disease outbreaks. Often, this technology can allow epidemiologists to identify the source of the contamination and thus what foods may need to be recalled. Describe the molecular principles underlying RFLP and how they enable this type of epidemiological analysis.
What is a transcription factor? Give an example of a transcription factor and a possible role it may play in the aging process.
A mutagen such as ultraviolet (UV) radiation may cause. The genetic code AUG codes for. One strand of DNA molecule has the base sequence ATAGGT. The complementary base sequence on the other strand of DNA will be. Which of the following correctly desc..
1. rewrite the following so that they read from left to right in increasing order of organisation. tissue macromolecule
How might Lucy conceive a child with just one ovary and one uterine tube, widely separated on opposite sides of the pelvis like this.
Consider the amino acid lysine. Which of the followingbest describes where you predict this amino acid would be found?
Motor proteins use the energy in ATP to transport organelles, rearrange elemts of cytoskeleton during cell migration, and move chromosomes during cell division. Which of the following mechanisms is sufficient to ensure the unidirectional movement ..
How the double-stranded DNA breaks gets repaired?
q. if intelligent extraterrestrials visited earth 475 million years ago and after that again 300 million years ago at
Which process in eukaryotic cells will proceed whether O2 is present or not?
Write a Response paper about the article "Microsatellite Instability Generates Diversity in Brain and Sociobehavioral Traits" by Elizabeth A. D. Hammock and Larry J. Young.
If the mutation in the Saccharomyces cerevisiae is an ochre mutation (see introduction), what amino acids may be substituted into the polypeptide by a single base mutation?
The results of the cross were 129 red-eyed females, 132 red-eyed males, 88 white-eyed females, and 86 white-eyed males. Test these data by chi-square analysis, and determine if the results are consistent with Morgan's hypothesis that Drosophila ey..
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