Pcr amplify a gene,, You're trying to PCR amplify a gene,

Pcr amplify a gene,

Assignment Help Biology

Reference no: EM131003575

You're trying to PCR amplify a gene, but your gel shows that there are two bands instead of only one like you expected. You know for certain that there no homologous genes in the genome from which you're amplifying, so you assume that the issue must be coming from your primers or PCR conditions. Briefly describe 2 different strategies for solving the problem and how they work.

5. You have sequenced a human protein gene from genomic DNA. You would like to use E. coli as mechanism to express this protein. Identify and briefly describe two modifications you might have to make to make to your sequence in order to have successful expression.

6. Restriction fragment length polymorphism (RFLP) analysis is commonly used for tracing the relatedness of pathogenic microorganisms in food-related disease outbreaks. Often, this technology can allow epidemiologists to identify the source of the contamination and thus what foods may need to be recalled. Describe the molecular principles underlying RFLP and how they enable this type of epidemiological analysis.

Reference no: EM131003575

Questions Cloud

The nash equilibrium will be : In a simultaneous move game, if both charge the low price (LP) they each earn $5K. If both charge the high price (HP) they each earn $10K. If they charge different prices, the one playing LP earns $15K and the other earns $1K. The Nash Equilibrium wi..

Prepare the year-end journal entries to record income taxes : The enacted tax rate for 2011 and 2012 was 30%, but during 2012 new tax legislation was passed reducing the tax rate to 25% for the years 2013 and beyond.

Compute volume of the solid obtained by rotating the region : For the region bounded by y =2x2, y = 8, 0 ≤ x ≤ 2, please Sketch the region. Use the method of cylindrical shells to find the volume of the solid obtained by rotating the region about the y-axis.

Research how they reproduce : Research how they reproduce, and ID them using at least three phenotypic descriptions: tuberculosis bacterium, Streptococcus pnuemonae

Pcr amplify a gene, : You're trying to PCR amplify a gene, but your gel shows that there are two bands instead of only one like you expected. You know for certain that there no homologous genes in the genome from which you're amplifying, so you assume that the issue must ..

What are the abiotic and biotic factors of sphagnum moss : What are the abiotic and biotic factors of sphagnum moss?

How should the deferred tax amounts be classified : How should the deferred tax amounts be classified in a classified balance sheet?

The end of the second stage of interphase : What events mark the beginning and the end of the second stage of interphase? What happens in the cell during this stage?

Determine volume where the mixture will burst into flames : The mixture can be seen as an ideal gas. The mix has a startvolume of 2,3 m^3 and and a pressure of P =1 bar at the temperature 25 oC. The question: the mixture is compressed in a reversible adiabatic process. Determine the volume where the mixtu..

User Account

All Pages