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Techniques and Procedures - The graduate uses appropriate techniques or procedures for interacting with microorganisms in a clinic, laboratory, or community setting; utilizes basic laboratory techniques for identifying microorganisms; demonstrates proper laboratory techniques in microbiology, including sterile technique, staining techniques, aseptic technique, and chemical indicators; and recognizes types of culture media and when to use each.
Objectives:
Demonstrate proper aseptic technique when performing specified tasks in the laboratory.
Prepare a microscope slide for identification of a given microorganism.
Use a microscope to observe bacteria or blood samples in a laboratory setting.
Employ a specified bleach sterilizing technique in a laboratory setting.
Employ a specified preparation technique to observe a given bacterial morphology.
Employ a given direct or indirect staining technique in a laboratory setting.
To get isolated colonies on a plate, only about 300 cells can be in the inoculum. What will happen if the cell density of the inoculum exceeds this number?
Gina is a college sophomore on a tight budget. An analysis of her diet shows that she consumes less than the RDA for vitamin C and more than the RDA for thiamin. Does she have a vitamin C deficiency.
Estimate the consequence of a DNA mutation, within the first exon of a gene, that results in a change from TAT, which codes for Tyrosine, to TAA?
The genes for body color and wing size in Drosophila are on the same chromosome and forecast the ratios of the following phenotypes among the offspring,
If the hernia allele in Belgium terriers has a frequency of 0.09, what is the probability that a puppy you buy will develop this problem (the problem is expressed by the homozygous recessive condition hh)?
Unlike steroid hormones, signaling molecules that are large and/or hydrophilic cannot pass through the cell's plasma membrane and therefore must bind extracellularly to receptor molecules in the plasma membrane.
You are interested in improving the vitamin content of your plant of interest. Describe briefly the steps that you would take to clone a gene encoding an enzyme required for the vitaminbiosynthesis
Moniliasis is one of the most common opportunistic diseases in AIDS. What is the etiological agent of moniliasis and what is the other name of the disease?
The overall reaction of aerobic respiration of glucose is summarized as.
A geneticist makes a cross between two homozygous strains of maize with three different mutations. For symbols, you can use a capital to indicate the dominant allele.
When the F1 portion of the ATP synthase complex is removed from the mitochondrial membrane and studied in solution, it hydrolyzes ATP to ADP. Why does it notfunction as an ATP synthase?
The decomposition of N2O5 in CCl4 proceeds as follows: 2N2O5 --> 4NO2 + O2. The rate law is first order in N2O5. At 64 degrees Celsius, the rate constant is 4.82 x 10^-3 S^-1.
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