Nucleotide composition of strain

Assignment Help Advanced Statistics
Reference no: EM132669521

You are studying the variation among natural bacterial isolates from thermophilic vents, where temperatures routinely reach 55°C (131°F). You compare the Tm of DNA from one of your isolated thermophilic bacteria with an E.coli lab strain, which grows at 37°C (98.6°F). To determine Tm you heat the genomic DNA to 100°C while taking OD260 readings every 20°C degrees.

  1. What is the definition of the Tm-?
  2. Which has a higher absorbance at OD260, single stranded DNA or double stranded DNA?
  3. State the predicted Tm for each, as based on your expected results.
  4. Why do you predict this?
  5. What does this suggest for the nucleotide composition of each strain?

Reference no: EM132669521

Questions Cloud

Formation of gametes and ending : The primary goal of this system is the production of a new human being. Let's consider each step of that process, beginning with the formation of gametes
Analyzing the cultural messages and effects of artifact : Identifying and analyzing the cultural messages and effects of your artifact. Does your artifact say something implicitly or explicitly about a culture?
What are Anh regular tax and alternative tax NOL amounts : Included in the losses are $30,000 of AMT preferences. Given this information, what are Anh's regular tax and alternative tax NOL amounts
What is Gabriel and Emma AMT adjustment : Gabriel, age 40, and Emma, age 33, are married with two dependents. What is Gabriel and Emma's AMT adjustment for itemized deductions in 2019
Nucleotide composition of strain : You are studying the variation among natural bacterial isolates from thermophilic vents, where temperatures routinely reach 55°C (131°F).
What is the rna world hypothesis : What is the RNA World Hypothesis? What is the evidence to support the RNA world hypothesis? How could an RNA World have evolved into our world today?
How much investment interest expense can Yoon deduct : How much investment interest expense can Yoon deduct for regular income tax purposes for the year
Does the research design answer the research question : Does the research design answer the research question? Explain your rationale. Were the study sample participants representative? Why or why not?
What happens if iron levels in the body are too low or high : Choose one of the major minerals and one of the trace minerals from your reading as examples. What does deficiency look like for each? What does toxicity look

Reviews

Write a Review

Advanced Statistics Questions & Answers

  How housing characteristics are capitalized in housing price

In analysis and discussion section you should discuss how housing characteristics, neighborhood characteristics and school quality are capitalized in housing prices.

  Critical analysis of scholarly article

Scholarly writers are aware of their audience and base their writing on solid evidence rather than on assumptions and/or opinions. In addition, scholarly writers must also utilize a scholarly voice.

  Description of long term debtor

For the year ending June 30, 2008, the New Corporation has current assets of $275,000 and total assets of $900,000. It also has current liabilities of $100,000, equity of $200,000, and retained earnings of $300,000.

  Find the eigenvalues and eigenvectors

Find the eigenvalues and eigenvectors of [Km], where [Km] is the mth power of [K]. Find the normalized eigenvectors. Express [K] as [QηQ-1], where [η] is diagonal and [Q] is orthonormal.

  How much time in total will the cashier be idle

How much time in total will the cashier be idle and how many customers on average are waiting in line to pay?

  Managerial and financial accounting

What type of information does managerial accounting provide? What type of information does financial accounting provide?

  Question 1 determine the area of the shaded region z032 the

question 1. determine the area of the shaded region z0.32. the graph depicts thestandard normal distribution with mean

  What is distribution of the time till next epoch of n2

What is the distribution of the time till the next epoch of N2(t) and what is the probability that the next epoch of N (t) is an epoch in N1(t)?

  What is the probability that fisher wins the match

What is the probability that Fisher wins the match? Your answer should not involve any integrals. Hint: Consider the unending sequence of games and use (b).

  Enforcement of affirmative action

In describing the relationship of affirmative action and descriptive statistics, I said, "Affirmative action is the legal enforcement of policies, descriptive statistics is specific descriptive data derived from research.

  Review the learning resources and the media programs

Write a 2- to 3-paragraph analysis of your t test results for each research scenario and include the SPSS syntax and output.

  What is probability of a score falling

What is the percentage of scores falling between the mean and a raw score of 84 and what is the probability of a score falling above a raw score of 86?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd