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Mutations affect the amino acid sequences

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Reference no: EM131764338

Lab Questions

1. Translate the following sequence.

5' AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3'

2. Create one or more point mutations in this sequence.

5' AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3'

3. Translate your mutated sequence.

4. Now create frameshift mutation by adding one or more bases.

5' AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3'

5. Translate your mutated sequence.

6. Create frameshift mutation by deleting one or more bases.

5' AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 3'

7. Translate your mutated sequence.

8. Align the amino acid sequences that you determined for each of the four transcripts.

1 ­­­____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____

2 ­­­____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____

3 ­­­____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____

4 ­­­____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ ____

Post-Lab Questions

1. How does each of your mutations affect the amino acid sequences? Are the mutations missense mutations, silent mutations or nonsense mutations?

a. Point mutation?

b. Frameshift-insertion?

c. Frameshift-deletion?

2. What differences did you notice between the point mutation and the frameshift mutations?

3. Is it possible to determine the DNA sequence from the following amino acid sequence?: Leu Pro Arg. Why or Why not?

Reference no: EM131764338

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