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You have isolated a mutant form of actin that can bind to ATP but not hydrolyze it. Other than that change the overall structure of the mutant form of actin is, for our purposes, identical to the wild-type for of actin. You start with a solution of your mutant form of actin in which the concentration of your G-actin is 10 microM. You add excess of ATP and Mg++ and let the reaction reach steady state. At steady state you measure the amount of F-actin and find that 95% of the G-actin monomers are now contained in F-actin polymers. What is the critical concentration for your mutant form of actin?
After your solution in the previous problem reaches steady state you dilute it 10-fold with the same buffer containing ATP and Mg++ but no actin. You wait an amount of time that you expect that if any reaction could take place they would have reached completion. At that time, you measure the amount of G-actin and the amount of F-actin. What fraction of the actin at steady state will be G-actin and what fraction will be F-actin. Explain your reasoning
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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