Mixture of proteins examine by standard 2-d electrophoresis

Reference no: EM1383133

Suppose you are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 121,201; pI 8.22), protein B (Mr 55,438; pI 7.44), and protein C (Mr54,274; pI 10.54).

With the SDS PAGE, in what order did the proteins band, starting with the well and proceeding to the bottom of the gel? Explain why.
If the mixture is subjected to CM-matrix chromatography, what pH range should you use for the buffer and in what order will the proteins emerge from the column? Explain why.
Upon gel filtration of the mixture, protein A emerges from a G2000 Sephadex column at a Ve of 115 ml protein B emerges at 155 and protein C at 190 ml. Based on this observation and the 2-D electrophoresis results, what can you conclude about the structure of protein C? Explain.
Upon salting out with ammonium sulfate, if you use pH 7.0 for the buffer, in what order should the proteins precipitate? Explain

Reference no: EM1383133

Questions Cloud

How to update web pages using navy for unvisited link : The Marketing Director at Steel Solutions is unhappy that links on company Web page appear in blue. She has asked you to update Web pages to use navy for unvisited link.

Crash at a railroad crossing : Due to a crash at a railroad crossing, an overpass is to be constructed on an existing level highway. the existing highway has a design speed of 50 mi/h.

Determine how these pending issues will affect you : Looking ahead, speculate illustrate what the pending issues in business will be 15 years from today. Based upon your answer to the previous discussion, determine how these pending issues will affect you and illustrate what you can do to prepare.

What is the increase in the internal energy of gas : A ship sets out to sail to a point 182-km due north. An unexpected storm blows the ship to a point 158-km due east of its starting point. How far must it now sail to reach its original destination.

Mixture of proteins examine by standard 2-d electrophoresis : Suppose you are given a mixture of proteins that you examine by standard 2-D electrophoresis, with the following results for isoelectric points.

Describe the key differences between simulation models : Describe the key differences between simulation models and the models covered in previous modules, not only from the perspective of their applications, but also from the perspective of computing/solving the models.

What must the banking angle of the curves : An automobile club plans to race a 700kg car at the local racetrack. The car needs to be able to travel around several 155m radius curves at 87km/h.

Compute the amount of aggregate demand of price level : Use the values for the amounts of autonomous planned spending which is independent of the interest rate and the nominal money supply to derive the equation for the aggregate demand curve.

What is the expected value for the recessive phentotype : What is the expected value for the recessive phentotype of the cross Long versus Short and what is the Chi Square Sum (rounding to 3 significant figures) for Long versus Short cross?

User Account

All Pages