Mixture of four proteins of different molecular sizes

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You are required to separate a mixture of four proteins of different molecular sizes (from M.W. 6000 to 12000) using a Sephacryl-packed gel-filtration column.

a) How could you calculate the respective eluted volume (Ve) for each of the four proteins if NO dyes were included in the protein mixture? Please elaborate on your answer, you can include a figure if you like.

b) If you can add two more dyes to the mixture as indicators for the elution process, what will you add? Please provide the name and the rationale for using those two dyes.

Reference no: EM132439605

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