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Question: Suppose a protein of interest is known to bind the mitochondria to the ER. Suppose these proteins are small enough to fit into the vesicles and transfer to the Goldgi apparatus. A putative KDEL sequence similar to the one on Bip proteins has been identified. How could one determine whether the sequence is necessary for our 'protein of interest'? We do not have access to antibodies, but all lab equipment and necessary reagents are available at our disposal.
I understand how to make antibodies, and the gist of running subcellular fractionation and gels. Some assistance in figuring this out would be really appreciated.
how The sex of a human is determined
If a cube representing the cell is 5um on a side, compute the surface area to volume ratio, and explain why this is or is not a good size for a cell.
Describe how body combats an invading bactrum. Address both specific and nonspecific immunity and the cells that are involved in each process. Once that is done cross the alveolar membrane and trace your route out of the body through the nose.
I have 30µg of a primer, whose molecular weight is 600. Determine how much water should I add to get a stock primer concentration of 100µM?
How will you find out the transcription factor binding sites in the whole genome. You have isolated a novel transcription factor. How would you decide the genes regulated by transcription factor in the whole genome.
The performance of a seed can be determined by studying its germination and viability potential.give One of the most significant advances in seed testing.
Starting with a hydrophilic signaling molecule, explain the process that takes place with a G-coupled protein signaling pathway. Suppose that cAMP is your main secondary messenger.
Use information collected from visual comparison or the colorimetric measurements, what were the initial velocities for the uncatalyzed reaction, and those catalyzed by the purified acid phosphatase, and the wheat germ extract.
Diversity in tropical regions is generally greater than at high latitudes. Explain two different hypotheses that propose the mechanism for this pattern that remains a biological mystery
Based on the general permeability equation, net flux of solute across a cell membrane, Jnet (gm/sec), is proportional to the permeability coefficient, P,
Some wines are marketed as "sparkling" wines. Sparkling wines are considered so because of a dissolved gas that makes them fizzy. Concern what you know about the procedure of respiration to explain the type and origin of this gas.
You observe the following numbers of individuals of each genotype in a population, Determine the observed genotype frequencies in this populaiton?
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