Reference no: EM132489662
Methods of isolating bacteria in a pure culture
Streak Plate Method
1. What is the streak plate method allowing for?
2. Flame your loop before setting it down. Why?
3. What will be happen during incubation?
4. Why inverted?
5. How can you tell whether you touched only one colony and whether you have a pure culture?
6. Where isolated colonies occur will depend on?
Serial Dilutions and Pour Plates Method
7. What is the main advantage of pour plate method?
8. Does the pour plate use melt agar?
9. How serial dilution occur? Clarify this?
10. How to equate solid samples?
11. What is the purpose of using the vortex mixer?
12. What is the purpose of using stacking plates?
13. What is the reason for plating serial dilution?
14. What is the type of agar that used in this experiment?
15. What is the purpose of using water bath?
Disk Diffusion
16. What is the other name of disk diffusion test?
17. What type of nutrient medium that clinical laboratory recommended to use?
18. Why we swab in one direction without leaving gap?
19. What is the purpose of using the Antibiotics disk?
20. What is the purpose of using the ruler?
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Methods of isolating bacteria in a pure culture
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