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DNA replication in eukaryotes and prokaryotes starts when a complex of proteins binds to an origin of replication and separates the two strands of the double helix to form a replication bubble with replication forks at each end. In order for replication to proceed, the enzyme topoisomerase binds to the DNA immediately in front of the advancing replication forks.
A) Describe how the structure of a prokaryotic chromosome differs from the structure of a eukaryotic chromosome.
B) Explain why DNA replication cannot proceed to the regions of the chromosome unless topoisomerase binds ahead of each advancing replication fork.
C) Based on the mechanism of DNA polymerase, explain why each replication fork will have both a leading and a lagging strand.
D) Eukaryotic cells like ours require telomeres at the end of chromosomes in order prevent loss of important genetic information. Prokaryotes do not require telomeres. Propose an explanation for why telomeres would be important in eukaryote cells, but not prokaryotes.
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