Measuring bacterial population growth by spectrophotometry

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1.) A bacterium that is normally rod shaped suddenly acquires a mutation that makes the cells unable to produce the cytoskeletal protein MreB. What shape will these mutant cells have? How do you know?

2A.) What do you think might happen to the morphology (size and/or shape) of a rod shaped cell that cannot make an FtsZ ring at all?

2B.) Cells don't die immediately if they can't make an FtsZ ring, but they are very unhealthy. What makes these cells unhealthy? Hint: Think back to our discussion about the typical size of bacteria.

3.) When measuring bacterial population growth by spectrophotometry, most protocols instruct the experimenter to take optical density (OD) readings once an hour for part of the growth curve, and once every 20 minutes for a different part of the growth curve. Given your knowledge of bacterial growth dynamics, which comes first, the 1 hour or 20 minute readings? Why?

Reference no: EM132635511

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