User Account

All Pages

Making reaction

Assignment Help Biology
Reference no: EM1383786

Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase. Describe how to make the following reaction:

5mM MgCl2

1x buffer

1mM dNTP mix

10ng of primer 1

10ng of primer 2

1ug of Rna

0.25 units of reverse transcriptase

Final reaction 20ul

Reference no: EM1383786

Questions Cloud

Employers and employees have a unique relationship : Do you believe Pam is acting unethically? What about Dunder Mifflin? Explain please? Image text transcribed for accessibility: Employers and employees have a unique relationship
Test of a driver perception reaction time : A test of a driver's perception reaction time is being conducted on a special testing track with wet pavement and a driving speed of 55 mi/hr. When the driver is sober, a stop can be made just in time to avoid hitting an object that is first visib..
Epithelial tissues during primary cell culture : Explain why does depletion of Ca2+ from the medium through chelating agents promote isolation of cells from epithelial tissues during primary cell culture?
Universal functions of religion : Critically discuss the four universal functions of religion and the importance of each of these to human beings and social relationships. Can we have these relationships without religion?
Making reaction : Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase.
Explain application architecture and process design : Explain the application architecture and process design.Create the physical data flow diagrams. Use Microsoft Visio to make examples of flow charts and data flow diagrams.
What is the total area of the surface : What is the length of BF, in centimeters and how many exams has Jeff taken so far and what is the total area of the surface of the cube that is NOT red?
Operations reflect each of those management strategies : Identify MNEs whose operations reflect each of those management strategies. Then discuss the ways in which organized labor can possibly affect (or has affected) their pursuit of those strategies.
Tensions in suspended cables : The 20-kg mass is suspended from three cables. Cable AC is equipped with a turnbuckle so that its tension can be adjusted and a strain gauge that allows its tension to be measured. If the tension in cable AC is 40 N, what are the tensions in cable..

Reviews

Write a Review

Biology Questions & Answers

  Convert a proton gradient into atp

ATP-synthase is a large membrane-integral protein complex that produces ATP from ADP and inorganic phosphate. Describe the location of this complex in the cell; list its major parts and the subunits each part is made of. Describe how it is able to co..

  Question about hybrid crosses

Huntington's disease is expressed in individuals with at least 1-dominant allele, while albinism is expressed in individuals with homozygous recessive genotype

  Which genes would be expressed in a showy ornamental flower

According to ABC model of floral development, which genes would be expressed in a showy ornamental flower with multiple sepals and petals but no stamens or carpels.

  The yield coefficient based on glucose

What proportion of male children in a large family will finally develop baldness in a marriage where both husband and wife are heterozygotes with genotype HnHb?

  How genetic material is replicated

Eukaryotic cells are more complex than prokaryotic cells. Explain their differences, functions and the composition of each including how genetic material is replicated.

  How might you use radioactive isotope

Animals obtain energy during a sequence of chemical reaction in which sugar (C6H12O6) and oxygen gas (O2) are reactants. This process produces water (H2O) and carbon dioxide (CO2) as waste products. How might you use radioactive isotope to find out w..

  What does this suggest about the flow of energy

How do plant and animal cells metabolize energy in complementary ways? What does this suggest about the flow of energy and the recycling of elements through living organisms? Could we think of any other similar relationships.

  Ecology and evolution

Explain the three kinds of the mammals based upon how their young grows.Explain briefly the functions of sensory neurons, inter-neurons, and motor neurons in nervous system.

  Photosynthesis and cellular respiration

Explain three ways that the knowledge of metabolic processes photosynthesis and cellular respiration can improve your level of physical fitness.

  Offer a hypothesis to explain this behaviour

In mid afternoon, they are on it's under surface, each caterpillar hanging by a few legs. Offer a hypothesis to explain this behaviour. How would you test it.

  Define the bold words from the case study in apa format

About twice a year, one or two more birds from the neighbouring island arrive. What result do these new arrivals have. There are questions that follow the case study and the first one says to define the bold words from the case study in APA format. I..

  Abo blood types in humans

Suppose that an animal that has a system similar to ABO blood types in humans.  The ABO part is the same. In addition, they have a second independent gene that makes a mimic A protein

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

Submit Assignment

Academics

Major Subjects

Majors

Get In Touch

whatsapp: +1-415-670-9521

Phone: +1-415-670-9521

Email: [email protected]

TERMS & POLICIES

HELP & SUPPORT

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd