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Question: Assume you are creating a PCR reaction mixture with a total volume of 50µL. The primer set concentration is 1µl primer/10µl reaction volume. The plasmid stock solution concentration = 0.01µg/µl, of which you need 100ng.How much of each PCR reaction component will you add, given the following?5x Mastermix __________Primer set __________DNA template __________H20 _____________
What is contributing to the decreased alveolar ventilation. A 22-year-old man was in a motorcycle accident with resultant neck injuries that led to partial paralysis of the upper and lower limbs. Approximately immediately his chest felt heavy and he ..
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
For a given reaction, G = -4 kcal/mole and the energy of activation for the uncatalyzed reaction = 5 kcal/mole. You add an enzyme, after which the activation energy decreased to 3 kcal/mole.
Explain how black and white pattern on the male siamese cat arises. If the oceans warm a few degrees, what impacts can you see on the landmasses, biologic community, and human populations? Are these positive or negative changes? Why with reason.
Some wines are marketed as "sparkling" wines. Sparkling wines are considered so because of a dissolved gas that makes them fizzy. Concern what you know about the procedure of respiration to explain the type and origin of this gas.
Before this module evaluation you were very nervous. Consider a snack might help, you ate a big bowl of pasta and some bread a couple of hourse before you started this quiz.
Suppose that you are working with Drosophila and are studying genes, a, b, c, d, e, and f that are all linked in a region on a chromosome, but their order is unknown.
Discuss the similarities and differences between fungi and protists. How do both of these kingdoms differ from the kingdom Eubacteria?
Determine when a blood vessel bifurcates into two smaller vessels, the radii of the two smaller vessels add up to a larger cumulative radius than the radius of the original vessel.
Martha is maintaining her current weight by eating 2,500 kcalories per day. To lose 1 pound of fat per week, she would have to decrease her intake to how several kcalories per day. Considering the albedo of various surfaces how might temperatures var..
Illustrate the structures of the sun including the interior, surface and atmosphere and what type of science is done to come to these conclusions. How does the structure of the sun compare to structure of the earth.
If a population of algae-eaters experiences predation pressure from pike-cichlids which is the least likely to be observed in the algae-eater population over the course of many generations.
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