Making a pcr reaction mixture

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Reference no: EM1389261

Question: Assume you are creating a PCR reaction mixture with a total volume of 50µL. The primer set concentration is 1µl primer/10µl reaction volume. The plasmid stock solution concentration = 0.01µg/µl, of which you need 100ng.
How much of each PCR reaction component will you add, given the following?
5x Mastermix __________
Primer set __________
DNA template __________
H20 _____________

Reference no: EM1389261

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