Make the following working dilutions of alkaline phosphatase

Reference no: EM13889517

Make the following "working" dilutions of alkaline phosphatase (total 0.4 mL each) in microfuge tubes by diluting the stock preparation of 1.3 microgram/mL alkaline phosphatase. use the enzyme buffer (100 mM Tris (pH 8), 5 mM MgCl2 and 0.10 mg/mL BSA) for preparaing dilution.

Working AP concentration in microgram/mL	Volume stock 1.3 microgram/mL alk.phos.	Enzyme buffer (pH 8)
0	0 micro liter	400 micro liter
0.065
0.13
0.26

Reference no: EM13889517

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