Make a loss of function mutation in the lacy gene

Reference no: EM1397433

you decide that you want to figure out a situation where you see constant transcription of the lac genes but you want the amount of transcription to be low. which scenarios will give low transcription?

1. make a loss of function mutation in the operator of the lac operon and add glucose

2. make a loss of function mutation in CAP and add lactose

3. Make a mutation in the promoter of the LacI repressor and add glucose and lactose

4. make a mutation in the LacI repressor such that it cannot bind inducer but can still bind the DNA

5. make a loss of function mutation in the LacY gene

1. high

2. low

3. low

4. low

5. high

Reference no: EM1397433

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