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Lysozyme has two key catalytic residues, Glu35 with a pKa of 5.9 and Asp52 with a pKa of 4.5. If the pH optimum for this enzyme is 5.0, what does this tell you about the mechanism?
a. Asp 52 is more likely a nucleophile than Glu 35.
b. Glu 35 is more likely a general acid than Asp 52.
c. Both of these are true.
d. Neither of these are true.
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