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Antibodies: A. Consist of 2 heavy chains & 2 light chains held together by disulfide bonds. B. Can be used to detect proteins associated with their subcellular structures in living cells by indirect immunofluorescence microscopy. C. Cannot be used to affinity purify proteins because antigen-antibody complexes cannot be dissociated without denaturing the antigen. D. Cannot recognize an epitope as small as a single phosphorylated amino acid. E. (a) (b) (c) and (d) Indirect immunofluorescence microscopy utilizes: A. A fluorescently tagged primary antibody that binds by its Fc region to an epitope on its corresponding antigen. B. A fluorescently tagged primary antibody that binds by its CDR to an epitope on its corresponding antigen. C. A fluorescently tagged secondary antibody that binds by its CDR to the Fc region of a primary antibody bound by its CDR to an epitope on its corresponding antigen. D. A fluorescently tagged secondary antibody that binds by its Fc region to the CDR of a primary antibody bound by its Fc region to an epitope on its corresponding antigen. E. A GFP-tagged protein that is directly visualized in a living cell. How would you examine the localization of a chimeric GFP-fusion protein in a living cell using an epifluorescence microscope? The λexcite for GFP is 395 nm & λemission is 509 nm. A. Just view the cell at 395 nm as GFP is self-fluorescing. B. Just view the cell at 509 nm as GFP is self-fluorescing. C. Irradiate or illuminate the cell at 395 nm & view it at 509 nm. D. Irradiate or illuminate the cell at 509 nm & view it at 395 nm. The masses (kDa = kilodaltons) & protein:lipid ratios of three human plasma lipid transport particles are: VLDL (very low density lipoprotein particle) ~10 kDa; 1:100 LDL (low density lipoprotein particle) ~2 kDa; 25:100 HDL (high density lipoprotein particle) ~0.2 kDa; 90:100 Based on this information, which two methods could be effectively used in combination to separate these three different lipid transport particles from each other? A. Equilibrium density-gradient sedimentation and LC/MS-MS B. Equilibrium density-gradient sedimentation and ion exchange chromatography C. Gel filtration chromatography and differential centrifugation D. Differential centrifugation and equilibrium density-gradient sedimentation. E. Equilibrium density-gradient sedimentation and gel filtration chromatography
Approximately how many people in sub-Saharan Africa are infected with HIV? How many died in the last year?
Elevated levels of plasma glucose in diabetics uncontrolled are often associated with high LDL cholesterol, because this happens?
If a population of snails on the mainland has an allele frequency of p = 0.9 and the allele frequency on an island is p = 0.2, what will the allele frequency be on the island after one generation of migration with m = 0.1 ? Show your work.
A protein is a trimer, which you suspect may exhibit cooperative binding. Every monomer contains a single binding site for the ligand, A. To investigate this you get the this data shown with total=20uM.
A analyzer discoveres that the correlation coefficient between the exposure to a dangerous chemical and the growth of a certain species of butterfly is -.40.
A very important part of the paper is your own opinions and ideas, based on the research you have read. Indicate which sources you agree with and which you do not, and explain your answer.
In a prokaryotic cell, where would you expect to find enzymes that break down cellulose?
A cross is made between a leucine-requiring haploid yeast strain of genotype leu and a wild-type strain that can make its own leucine (leu+).
In rabbits, black is due to a dominant gene, B, and brown to its recessive allele, b. Short hair is due to a dominat gene, S, long hair to its recessive allele, s.
Describe the various explanations (evolutionary, anatomical, etc.) for why pollinators are often very specific to the plants they pollinate.
The wild type fruit fly, Drosophilia melanogaster, has straight wings and long bristles. Mutant strains have been isolated that have either curled wings or short bristles.
In a population of 100, 25 individuals are homozygous dominant. How many individuals would be expected to be homozygous recessive?
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