Reference no: EM132383934
Lab - Spectrophotometric Quantitation of β-Carotene
1. Using your data for the standard β-carotene assay, construct a standard curve by plotting A449 (y-axis) vs. mg/ml β-carotene (x-axis). This may be done by hand using proper graph paper (20 squares per inch) or by computer. Tabulate your data on the back of your graph.
2. Give a sample calculation (with units) for the final β-carotene concentration in one of the standard tubes (B-E).
3. Calculate the mg of β-carotene per g carrot (mg β-carotene/g carrot). Indicate your method neatly and clearly, including units. (The following steps will helpful).
a. Calculations for ε from standard curve.
b. Concentration of β-carotene in diluted extract.
c. Concentration of β-carotene in concentrated extract.
d. Total mg β-carotene extracted.
e. mg β-carotene/g carrot.
Attachment:- Lab Assignment Files.rar
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