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1) In order to separate the RNA you majorly utilize a protein denaturant solution of the guanidinum salts. These chaotropic solutions denature by disrupting the H- bonding. The solutions also consists of a reducing agent i order to denature disulfide bonds. The final composition of the solution is : 4 guanidine isthiocyanate (GITC), 25mM sodium citrate, pH 7.0 , 0.5%(w/v)SDS, 100mM β-mercaptoethanol. You have the guanidine isothiocyanate powder(mol.Wt.118.16),1 M Na Citrate, pH 7.0, powder SDS and b-mercaptoethanol(density 1.12 g/m L, mol. Wt.78.1). State how would you make up the 100 mL of this solution?
Under constant erosion and weathering, the pyrites leech large amounts of sulfuric acid (H2SO4). The spoil banks are also mixed with large quantities of basic limestone and clay carbonates. What have to occur over time.
In an experiment you are studying a neuron that receives synaptic inputs from two other neurons: one from neuron A and one from neuron B. Illustrate a separate graph for each value of Vm.
What is an advantage of using GFP fusions to visualize specific proteins, instead of staining cells with fluorescently labelled probes that bind to the target protein.
The pH of the inner thylakoid space had been measured, as have the pH of the stroma and the cytosol of a particular plant cell. Why will this cause the pH in the thylakoids to be lesser than the pH in the stroma.
For in case, it is well established that in presynaptic neurons, the elevation of cytosolic calcium triggers the fusion of exocytotic vesicles that contain neurotransmitters to the plasma membrane.
State how the amonio acids sequence you obtained from the gene fragment for botana curus compares with the sequence for other three species.
List and describe at least three cellular features of bacteria that could be targeted to inhibit or kill bacterial pathogen. Which is a significant flaw in this experimental set-up.
A 40 year old woman was admitted into ER with fever, chills, and a dry cough with little sputum. Epidemiologists isolated pathogen in the cooling system of the air conditioning unit.
Frogs are extremely sensitive to acid rain. How might this information be used to investigate your observation about the trees dying in the forest.
Why or why not explain with reason? Work the genetics problem predicting the frequencies of the versions of this trait among their prospective children.
Compute the change in energy of an atom that emits a photon of wavelength 2.21 meters. (Planck's constant is 6.626 x 10-34 joule seconds; the speed of light is 2.998 x 108 m/s)
From a cell's point of view, what is the reason of a cell, and what is the purpose of a gene? From a gene's point of view (according to Dawkins), what is the purpose of a cell and what is the purpose of a gene.
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