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A student attempts to transform a population of E. coli with a pBluescript plasmid. How do you advise him to identify cells that have the plasmid? Answer Plate the cells on a medium containing X-gal. Cells with plasmids will turn blue. Plate the cells on a medium with ampicillin. Cells with plasmids will grow. Plate the cells on a medium with the restriction enzyme EcoRI. Cells with the plasmid will grow. Infect the cells with bacteriophage T3. Cells with the plasmid will transcribe the inserted gene and not be able to grow. Isolate individual colonies and prepare DNA from each. Size the DNA on an agarose gel to see if plasmids are present.
Plants often produce waxes on the surface of their leaves to prevent dehydration and protect against insects. What structural feature of waxes makes them more suitable for this task than carbohydrates or proteins.
What are the advantages of having a gene regulated by both positive and negative control, rather than by just one or the other?
Utilize darwin's theory of evolution by natural selection to explain how a group of ancient mammals couple be the ancestors of a major new group of mammals, the cetaceans.
Suppose as you are working in the lab, you obtain a major career-defining result. You have discovered a new TLR, which recognizes either a bacterial or viral antigen.
Molecular Weights as determined by SDS-PAGE & NATIVE PAGE GEL How do the two masses compare with one another as determined by the two methods?
Glucose and sucrose showed a reaction. Lactose and galactose didn't. Why?Glucose and sucrose easily made alcohol.
The water appears to be contaminated with radioactive waste. This has resulted in a highly accelerated mutation rate of 1/100 "A" alleles mutating to an "a" allele every generation.
Suppose that an animal that has a system similar to ABO blood types in humans. The ABO part is the same. In addition, they have a second independent gene that makes a mimic A protein
Assume you have a protein solution of concentration 3 M. You require to make a series of six dilutions of 300 mM, 30 mM, 3 mM, 300 mu M, 30 mu M, and 3 mu M.
You have an F- strain of E. coli that is resistant to strepto- mycin (Str(r)) but requires the following amino acids for growth on minimal medium: arginine, cysteine, methionine, phenylala- nine, and proline.
what hormones are produced by the theca and granulosa cells of a maturing or pre-ovulatory follicle?
Suppose that you are a scientist sequencing the genomes of organisms. You start two new projects, one sequencing the genome of an amoeba.
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