Isolating aspartame transmigrate

Reference no: EM13847378

You have a mixture of 3 proteins, listed in the table below with their relevant properties. You areinterested in isolating aspartate transaminase using ion exchange chromatography with DEAE resin. Giventhe values in the table below: 1) what pH would you use in order to ensure that aspartate transaminaseprotein binds to the column and that the others do not? Assume that your efforts are successful -- 2) howwill you elute aspartate transaminase from the column? Choose the correct conditions from the list below.

Protein pIAspartate transcarbamylase 9.8

Myoglobin 7.0

Aspartate transaminase 5.0

A) 1) Initial pH = 10.0;2) lower pH to 6B)

B)1) Initial pH = 8; 2) raise pH to 10

C) 1) Initial pH = 4; 2) raise pH to 5 or higher

D) 1) Initial pH = 6; 2) lower pH to 5 or less

DEAE is postive so attracts negative. if ph<pi means negative charge so initial ph would be more negative than pi then, raise ph to to neutralize. that's why I chose C but the answer is D. Why? The Pi's are the numbers beside the enzymes. I have a test this afternoon please respond quckily if you can

Reference no: EM13847378

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