Reference no: EM132669388
Read the Introduction to the photosynthesis lab section by section as you complete the following questions.
1. Write the summary chemical equation for photosynthesis.
2. Photosynthesis is divided into two processes.
A. What happens during the light reactions?
B. What is the principal photosynthetic pigment involved in the light reactions?
C. What other photosynthetic pigments are involved in the light reactions?
D. What happens during the Calvin cycle?
3. In Part I: Effect of Light Intensity on the Rate of Photosynthesis, develop a hypothesis for this experiment. Be sure to refer to the Scientific Method Lab about developing a good hypothesis.
Hypothesis:
4. In Part II: Separation of Chloroplast Pigments by Paper Chromatography,
A. What is the purpose of paper chromatography in this experiment?
B. What is the independent variable in this experiment?
C. What is the dependent variable in this experiment?
5. In Part II:
A. What is the equation for calculating the Rf value?
B. Refer to the diagram. Which of the 3 labels is the same as the origin in the Rf equation above?
C. You will use "Alternative Procedure for Transferring Pigments onto Paper" in the box below the diagram of the chromatography set up. Read the procedure. What could you use besides the handle of a pair of scissors to transfer the pigment onto the chromatography paper?
6. In Part III: Absorption Spectrum of Chloroplast Extract
A. What is the purpose of using a spectrophotometer in this exercise?
B. What is the independent variable in this experiment?
C. What is the dependent variable in this experiment?
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