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Phenol Red (PR) Broth is a differential test medium prepared as a base to which a carbohydrates is added. Included in the base medium are peptone and the pH indicator phenol red. We will inoculate PR Glucose Broths with three organisms to determine their fermentation characteristics.
Q1. Suppose you innoculate a PR broth with a slow-growing fermenter. After 48 hours, you see slight turbidity but score it as (-/-). Is this result a false positive or a false negative? Is this a result of poor specificity or poor sensitivity of the test system?
Q2. Early formulations of this medium used a smaller amount of carbohydrate and occasionally produced false alkaline (pink) results after 48 hours. Why do you think this happened? List at least two steps, as a microbiologist, that you could take to prevent the problem.
In a particular squash plant, white fruit is dominant to yellow fruit.A white fruited plant is crossed to a yellow fruited plant.Whe the seeds from this cross are planted.
On a normal ordered chromsome, two loci, a and b, lie 15 map units apart on the left arm of a metacentric chromosome. A third locus, c, lies 10 map units to the right of b on the right arm of the chromosome.
Primary transcripts produced by RNA polymerases are generally faithful copies of the DNA template. However, most final/functional forms of these RNAs vary substantially from the primary transcript.Give an instance of an RNA class.
We sometimes discover nematodes of unknown species in the digestive tract of bluegill that we dissect. Are these worms parasites or commensals?
How does inefficient transfer of energy among trophic levels result in the typically high endangerment status of many top predators.
Assuming that the distance between the corneal surface and the retina is 5 cm in an ostrich and that the refractive power of the ostrich's lens is 5 diopters.
In a germination experiment the germination rate and timing of wild radish (Raphanus raphanistrum) and crop radish (Raphanus sativus) were measured.
Which receptors were monitoring the drive, still the conscious part of your brain was asleep? Explain the proper answer.
What is the significance of adenine, thymine, guanine, and cytosine in the structure of a DNA molecule.define how these four nucleotides bond.
The matrix used in experiment has a fractionation range 20000 to 5x10^6 Daltons. Would you see this matrix purify a plasmid that had a molecular weight.
An amateur horse breeder has acquired four new bay-coloured mares (female horses) with uncertain pedigree. What are the probable genotypes of the mares purchased.
Discuss how does the cardiovascular system adapt with chronic endurance training to potentiate greater effects that you described in question 1?
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