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In your search for mutants of "your favorite gene" (yfg) youidentify a mutant which you believe to be a transposon insertion. At your disposal you havea point mutant of yfg. How can you distinguish this potential yfg transposondisruption from a nontransposon mutation?Which cloning vectors are best suited for the production of genomicDNA libraries with an insert size of 200Kbp? Describe one vector: What are theimportant features of the vector? What purpose does this vector serve in molecularbiology and genetics?Successful sequencing runs may obtain error free sequences for upto 1000 base pairs. Cosmid vectors have inserts up to 45000 base pairs. Suggest astrategy to obtain a complete sequence of a cosmid insert.
Why do you thinkthat Arsenic has been a favorite with poisoners over the centuries? A worker wascleaning out asbestos from a ceiling in the school. He was wearing the proper protection against inhalation, but some of itfell onto the skin of his arm. ..
Leon hopes to become an ichthyologist (fish biologist). What category of organisms does Leon hope to study?
Sanger-based sequencing needs very specific DNA and primer concentrations for optimal performance. For template DNA like plasmids, DNA needs to be at a concentration of 50ng/ul in 10 ul.
You put a cuvette into a spectrophotometer and obtain an absorbance reading of 2.4. How much light is actually reaching the detector?
The very simple genotype-phenotype relationship of dominance, codominance and recessiveness has been complicated by issues of penetrance and epistatsis.
Explain the how Natural Killer Cells and Cytotoxic T-cells recognize that a cell is infected with a virus and kills the infected cell and how antibodies recognize and neutralize viruses or target viruses for destruction.
Discuss and define late or delayed postpartal hemorrhage. What is the usual cause of this type of hemorrhage? List the symptoms.
On what bases would a physician (with the help of a laboratory technician) select an antibiotic to use in treatment of a disease?
After reading the article titled, "Epulopiscium fishelsoni, Big Bug Baffles Biologists!", use your knowledge about the limits to cell size and speculate as to why these cells can grow so large.
Identify three features of the digestion of fat that help to explain why Richard doesn't consume fat if an immediate source of energy is required.
With these anatomical features in mind, do research to find an angiosperm species that has structural modifications to leaves, stems, roots, or flowers that result in adaptive functions. For example, a California Barrel Cactus has spines in place ..
Explain the principle and advantage of using a rapid identification system, such as the Enterotube. How would you rate the Enterotube system with conventional bacteriological procedures to identify members of the Enterobacteriaceae family? Why?
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