In the study of biochemical processes

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Reference no: EM13703528

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2, often abbreviated as Tris. At 25 °C, Tris has a pKb of 5.91.
The hydrochloride of Tris is (HOCH2)3CNH3Cl, which can be abbreviated as TrisHCl.
What volume of 10.0 M NaOH is needed to prepare a buffer with a pH of 7.79 using 31.52 g of TrisHCl?
A: 6.7 mL
The buffer from Part A is diluted to 1.00 L. To half of it (500. mL), you add 0.0200mol of hydrogen ions without changing the volume. What is the pH of the final solution?
A: pH = 7.28
What additional volume of 10.0 M HCl would be needed to exhaust the remaining capacity of the buffer after the reaction described in Part B?

Reference no: EM13703528

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