Immobilized on the surfaces of nonporous polymeric beads

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Reference no: EM13252673

Consider an enzyme that is immobilized on the surfaces of nonporous polymeric beads.

The enzyme converts substrate S into product P with the following Michaelis-Menten parameters:

Km = 100 mg/l, (Vm)' = 0. 3 mg/(cm2 sec).

When the beads are placed in a stirred enzymatic reactor containing a solution with a bulk substrate

concentration of 100 mg/l, the observed reaction rate is 0.15 mg/(cm2 sec).

What is the value of the Damkohler number for the reactor?

What is the value of the substrate concentration at the bead surface?

Justify your answers and show work.

Reference no: EM13252673

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