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You have identified a strain of mutant E. coli that constitutively expresses from the lac operon. Considering that this is a mutation in a gene that encodes for a protein repressor, how would go about identifying the defective gene in the mutant strain? (For this question, genome sequencing is not an option)
Describe how the hypothalamus controls secretion from the pituitary gland. Be specific regarding circulation and communication between the two regions.
how to Calculate the fraction of Vmax. For an enzyme which obeys Henri-Michaelis-Menten kinetics.
A 10.0g sheet of gold with a temperature of 18C is laid flat on a sheet of iron that weighs 20.0g and has a temperature of 55.6C. Given that the specific heats of AU
Mycoplasmas are the smallest living cells. They are spherical and have a diameter of about 0.33 μm (radius of mycoplasmas = 0.165 μm)a.
Consider the following reaction sequence S + E (ES)1 (ES)2 --> P + E The first reversible reaction has forward and reverse rate constants of k1 and k2; the second reversible reaction has forward and reverse rate constants of k3 and k4; the thi..
At another locus, sepia eyes are inherited as an autosomal (non sex linked) recessive. The presence of the white eye phenotype (x superscript w x superscript w in females, x superscript y in males) masks sepia phenotype, so regardless of the sepia ge..
Discuss how many ways can these chromosomes be assorted into gametes and how many different chromosome combinations are possible when crossing two unrelated flies?
You have 0.05 mL of an undiluted culture at a concentration of 3.6 x 106 CFU/ml. You then add 4.95 mL sterile diluent. what is the dilution, and what is the final concentration of the cells?
Birds and mammals have a four-chambered heart, with two ventricles and two atria, but other modern reptiles have a three-chambered heart, with just one ventricle. Paleontologists debate whether dinosaurs had a typical "reptile-like" heart or a "birdl..
You are given a stock solution of EDTA at a concentration of 5mM, how would you dilution of EDTA with a final concentration of 5mM in a final volume of 250mL?
Substrate-level phosphorylation occurs within the metabolic pathway where sufficient energy is released by given chemical reaction to drive the synthesis of ATP from ADP and phosphate. In which the metabolic pathways are substrate-level phosphorylati..
A section of the E. coli genome that encodes the pheM protein. Write out and label: the reverse complement, the mRNA sequence.
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