Reference no: EM133671118

Assignment: Regulation of flagellar regeneration in Chlamydomonas

This is the second lab project for the course.

I. We will go over the rationale for the project, and in lab, we will learn how to stain and identify the flagella of Chlamydomonas reinhardtii.

1) Background information for the project: the file is add
2) Lab Protocol for 2/28: the file add

II. We will evaluate whether transcription and/or translation is required for flagellar regeneration (this is the actual experiment we are doing).

III. We will either complete the lab from 3/6 or review a paper related to the lab experiment

You will be working in groups for this lab. Each group will have ONE lab notebook file (electronic) and for each day we are in lab, your group needs to do the following:

1) Identify the purpose of the lab activity (this can be at most one-two sentences)

2) Write the methods that you used during the day, even if the methods are identical to what I hand out. Any changes you make to the methods also need to be recorded here.

3) Summarize the results of the day's activity. For this lab, this will include observations and measurements; data for the experiment on 3/6 can be summarized in a chart (I will provide a template for that chart format)

4) Each day needs a separate heading with the date

5) The notebook needs to include the names of the group members.