Identify possible problems with probe design

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A fellow researcher has designed probes for a PCR experiment. They ask you to review their work prior to submitting the sequences to the vendor. Based upon your knowledge of primer design provide feedback. Identify 2 possible problems with their probe design.

Forward (5)CCCTAGCAAACGTAGGG(3)

Reverse (5)CACGTTTGAATTT(3)

Reference no: EM132063874

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