Reference no: EM133418146

Question: You are a student worker in my laboratory. We have samples from the following animals or cell cultures, but regretfully you labelled the sample containers with non-permeant ink, and the ink is no longer readable for any of the sample containers.

Rooster cultured leydig cells, (This consists of the pure cells isolated from a sexually mature and active rooster as well as the cell culture media the cells are growing in. The media has all the necessary things to support normal leydig cell function).

Rooster cultured sertoli cells, (This consists of the pure cells isolated from a sexually mature and active rooster as well as the cell culture media the cells are growing in. The media has all the necessary things to support normal sertoli cell function).

Ralph a sexually active boar whose semen is collected and used for artificial insemination.

Simon a rooster that receives 15 hours of light each day and is fed a highly nutritious diet that supports optimal reproduction.

Tim is a rooster that receives 15 hours of light each day and is fed a highly nutritious diet that supports optimal reproduction. The activity of Tim's 5 alpha reductase is 10 times more active than normal and as a result his blood testosterone levels are not detectable.

Nancy is a hen, and for the past 5 days she produced an egg at 7am every day.

Olivia is a hen that produced an egg at 7am on day 1, no egg on day 2, an egg at 9am on day 3, an egg at 9 am on day 4, and no egg on day 5 (which is today).

Penny is a hen, and she laid an egg at 9am, 10am, 11am and 2:30pm on days 1, 2, 3 and 4, respectively. She did not lay egg today (day 5).

For all 3 hens the lights come on at 6am and go off at 9pm each day. Remember that after laying an egg, a hen can ovulate its F1 follicle within 30 minutes. Once ovulated, this ovum will be part of a completed egg that is laid about 24 hours after ovulation.

The graduate student informs you that the cell cultures have to be positively re-identified without a mistake. The laboratory has the necessary reagents to complete Western blots that will measure any protein present/produced by Sertoli or Leydig cells. In addition, the laboratory has the regents to measure any secreted hormone in the media. Limiting your answer to things covered in lecture identify 4 molecules (proteins or hormones) that you will measure, and whose absence or presence will allow you to identify the respective cultures