Identify a human homologue of the yeast protein

Assignment Help Biology
Reference no: EM133199245

A genetic screen undertaken in budding yeast has identified a gene whose product encodes a protein kinase that controls the transition of cells from G2 into mitosis. By sequence comparison, you identify a human homologue of the yeast protein, which you name PK1. You now wish to explore the regulation and function of PK1 in cultured human cells.

You wish to study PK1 activity in synchronous populations of cells that are in different stages of the cell cycle

You firstly treat the cells with a named drug in order to synchronise them in a specific stage of the cell cycle.

Having treated multiple plates of cells with the drug, you then wash out the drug and incubate each of the plates for different periods of time (from 0-24 hours)

Cell extracts are prepared for each plate of cells. These are analysed by SDS-PAGE and Western blotting using an antibody that only detects the active (phosphorylated) form of PK1.

At which stage of the cell cycle would you expect the strongest signal for active PK1 to be observed? Briefly explain why this would be the case

Reference no: EM133199245

Questions Cloud

Identify marketing technique you will use for each priority : IHP 510 Southern New Hampshire University. Identify marketing technique you will use for each priority (use one traditional, one social media, and one other).
Function of neurotransmitters : University of Phoenix-Create a pamphlet with pictures and graphics in which you. Explain the role and function of at least 3 neurotransmitters.
Describe specific mechanisms of phototransduction pathway : Phototransduction in rod cells can be extremely sensitive to light. Dark-adapted rod photoreceptor cells can respond to single photons, that is a single activat
Determine the subcellular localisation of gf1 : To investigate the secretory defect, you decide to use immunofluorescence microscopy to determine the subcellular localisation of GF1 within the SD1 and NC cell
Identify a human homologue of the yeast protein : A genetic screen undertaken in budding yeast has identified a gene whose product encodes a protein kinase that controls the transition of cells from G2 into mit
Does the review have a self-assessment component : JWI 520 People Management Performance Reviews Discussion Assignment, Strayer University - Does the review have a self-assessment component
How the cell goes from genotype to phenotype : Draw a picture with a description explaining how the cell goes from genotype to phenotype.
Evaluate the macro environments and competitive environments : You'll want to consider what changes Priceline should make and how Jeffrey Boyd should approach the upcoming challenges in the external environment.
Explain which eukaryotic organelle : Explain which eukaryotic organelle was discussed in Unit 2's attend discussion about genetically modified people in the future.

Reviews

Write a Review

Biology Questions & Answers

  Immune response to infectious disease

It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  Plastic as a soil stabilizer

Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.

  Principles of microbiology

This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.

  List the biologic functions

Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?

  Biologic function relationships

Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.

  Case study on patient in the haematology laboratory

Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.

  Use of pcr and genetic approaches in biotechnology

The use of PCR and genetic approaches in biotechnology

  Describe the role of this enzyme in honey

Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.

  Genetic problems

What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?

  Prepare an essay on nosocomial infection

Prepare an essay on nosocomial infection.

  Monitoring and recording the blood pressure

To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd