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A genetic screen undertaken in budding yeast has identified a gene whose product encodes a protein kinase that controls the transition of cells from G2 into mitosis. By sequence comparison, you identify a human homologue of the yeast protein, which you name PK1. You now wish to explore the regulation and function of PK1 in cultured human cells.
You wish to study PK1 activity in synchronous populations of cells that are in different stages of the cell cycle
You firstly treat the cells with a named drug in order to synchronise them in a specific stage of the cell cycle.
Having treated multiple plates of cells with the drug, you then wash out the drug and incubate each of the plates for different periods of time (from 0-24 hours)
Cell extracts are prepared for each plate of cells. These are analysed by SDS-PAGE and Western blotting using an antibody that only detects the active (phosphorylated) form of PK1.
At which stage of the cell cycle would you expect the strongest signal for active PK1 to be observed? Briefly explain why this would be the case
BIO 100 - Grand Canyon Human Population - How do diseases affect the population? Can you think about any diseases that has affected the human population?
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What do perceive to be a the better options intreating chronic pain prescription opioid medication or medicinal marijuana?
While in Europe, Rizal experienced a number of things that contributed more to his desire for a better Philippines. In a way, Padre Burgos' instruction for youn
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Most people with diabetes avoid eating foods with a lot of sugar. However, people with Type 1 diabetes sometimes need to eat snacks
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What does information suggest about the transmission.
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What effects are produced by an enzyme on the general reaction below? The rate constant for the forward reaction (K1) increases
This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment's questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The inte..
Paula has a sore throat. Her physician ordered a differential blood cell count and here are the leukocyte results per cubic millimeter: Total 15,000; mature neutrophils 13,000; 1,500 lymphocytes, 300 monocytes, 150 eosinophils, 50 basophils.
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