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Idea of the types of microorganisms

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You have isolated pure cultures of microorganisms from a clinical sputum sample. To get an initial idea of the types of microorganisms you isolated, you prepared the cultures on slides for light microscopy and subjected them to Gram staining. Upon examination of the slide, you found out that nothing can be seen on the slides. Assuming that all Gram stain solutions were freshly prepared and the microscope worked properly, what do you think went wrong? List two reasons.

Reference no: EM132735419

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