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1. Make a graph that shows how absorption for each pigment varies as a function of wavelength of light. Be sure your figure shows how absorption changes as a function of wavelength for all four pigments tested, plus the total pigment. All of the absorption spectra must be plotted on one graph (do not plot separate graphs for each pigment).
2. Hypothesize about the colors of light that chlorophyll pigments will absorb to some degree, based on the color of light they reflect (green).
4. Paper chromatography is used to separate pigments extracted in acetone from fresh spinach. Explain how differences in polarity are used to separate the pigments in this chromatography experiment?
5. A pigment solution contains compound A with 4 polar groups and compound B with 2 polar groups. You plan to separate these compounds using paper chromatography with a nonpolar solvent. Predict the location of the two bands relative to the solvent front. Explain your answer.
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