Hybridization to detect mrna

Assignment Help Biology
Reference no: EM1382116

Question: Assume if you are performing in-situ hybridization to detect mRNA, why do you use the antisense strand? Are you using RNA or DNA as a probe for mRNA? My probe is coming from a plasmid that must be digested by restriction enzymes.

Reference no: EM1382116

Questions Cloud

Formal and informal leaders : Critically discuss how people are formally and informally organized within your organization. Critically discuss who in your organization are the formal and informal leaders.
Evaluating the purpose of a message : List three helpful questions to ask in evaluating the purpose of a message. Give examples to show how those questions are helpful.
Explain main points to write in the communications plan : Recognize the main points you would write in the communications plan at this point in project. What suggestions would you give your project team to aid it manage user expectations?
Interaction using electronic channel : Explain how you could have changed your communication behaviors to make the interaction more effective while still using the electronic channel.
Hybridization to detect mrna : Assume i f you are performing in-situ hybridization to detect mRNA, why do you use the antisense strand? Are you using RNA or DNA as a probe for mRNA?
Calculate the quotas redistributive effect : Calculate the quota's redistributive effect, consumption effect, protective effect, and revenue effect and what is the overall welfare loss to Venezuela as a result of the quota?
Gender and racial diversity in organization : Illustrate out the degree to which there is gender and racial diversity in your organization. More specifically, you are to examine the degree of diversity of the people in the "leadership" positions relative to the rest of the workforce.
Compare changes in the carbon cycle : Compare changes in the carbon cycle to that of the nitrogen cycle on human health. Create a conceptual graphical model for how changing the carbon cycle is likely to impact human health.
We-versus-me orientation : Apply the communication competence model to the jury in the movie. Concentrate on the We-versus-Me orientation, skills and knowledge, appropriateness and effectiveness, sensitivity, commitment, and ethics.

Reviews

Write a Review

Biology Questions & Answers

  Explanation of phenomenon

Some malignant tumors remain localized and encapsulated, at least for a time; an illustration is carcinoma in situ in the ovary or breast. Share your thoughts to describe this phenomenon.

  Identify both selective and differential nature of organism

Describe how one media can identify both the selective and differential nature of the organism. What components of PCRs require to be changed in order to identify genetically modified meats or milk? Generally give details why those changes need to be..

  What other behaviours may have an impact on evolution

Elucidate how at least three organ systems are affected by this increased physical activity and discuss interactions among these systems.

  How organismal respiration and cellular are interconnected

How organismal respiration and cellular respiration are interconnected. While you visit him, his teeth are chattering, his skin is cool and clammy to touch, and he complains of feeling cold even though his room is quite warm. Elucidate his symptoms.

  Why the accumulation of lactate cease after o2 is added

Glucose is experimentally synthesized with C-14 at carbon 1, and used as a substrate for aerobic metabolism. Where is the label at the end of glycolysis.

  Describe a signaling pathway deregulated in cancer

Describe a signaling pathway deregulated in cancer that involves pRb and p53 as players.  Have any advancements been made in the understanding of these pathways since the provided review was written?

  Make sense in terms of chargaff''s rules

How would you interpret this result? How might such a phage replicate its DNA.make sense in terms of Chargaff's rules.

  General locations in water soluble globular proteins

Phenylalanine residues are often found in what general locations in water soluble globular  proteins?

  Question about consecutive crosses

Two consecutive crosses, with the first between pure-breeding parents that differ in two phenotypes to create an F1 generation, and the second in two F1 progeny to create an F2 generation.

  Organisms recognize others as members of their own species

Brown eyes (B) are dominant over blue eyes (b) in humans. If a brown-eyed man marries a blue eyed woman and they have three kids, and two of the three have brown eyes and other has blue, what would be the punnett square indicating so look like.

  Alga has invaded waters of california

The U.S government has placed this strain of C.taxifolia on noxious weed list, which means that any possible source of the contamination of the weed will be highly restricted. Shipments that contain any kind of C.taxifolia and pass through an area wh..

  What is the function of science and technology in society

What is the function of science and technology in society. If you are in an airplane encountering turbulence and you start feeling sick to your stomach, would closing your eyes help and why.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd