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How would you make up 10 ml of the stock pcr

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Reference no: EM131976441

How would you make up 10 mL of the stock PCR buffer from the following stock solutions?

  • 1 M Tris HCl pH 8.3
  • 2 M KCl
  • 183 mM MgCl2
  • 0.12 %(w/v) Gelatin
  • 100 mM for each of the dNTPs; dATP, dGTP, dCTP and dTTP

The stock PCR buffer contains:

  • 12.2 mM Tris HCl (pH 8.3),
  • 61 mM KCl,
  • 1.83 mM MgCl2 
  • 0.0012% (w/v) gelatin,
  • 244 μM dNTPs

Reference no: EM131976441

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