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Phenyl-Sepharose resin consists of a phenyl group attached to a matrix of agarose. Suppose you need to use this chromatographic method to separate a mixture of polypeptides consisting of polyalanine, polyserine, polytryptophan and polylysine. Would you apply these polypeptides in a buffer containing high ionic strength or low ionic strength? What buffer would you choose (choose from one of "Good's buffers" (find a list online)? How would you elute the polypeptides, and what would be the order of elution?
why the plasma membrane exists in all living things.
A microbiologist must culture a patient 's feces for in testinal (enteric) pathogens. Which of the following meida would he likely use. What is the purpose of resazurin in reducing media.
Consider Mendel's cross of pea plants with smooth, yellow seeds with pea plants with green, wrinkled seed that was reviewed in the Introduction. Why is immunogold electron micrography useful?
Genes A, B, and C are linked on a chromosome and found in the order A-B-C. Genes A and B recombine with a frequency of 10 percent, and genes B and C recombine at a frequency of 25 percent.
The 2.1kb long insert was excised from the source DNA using NotI. The insert fragment has 2 SphI restriction sites. One of these located 1.3kb from 1 end of fragment and other is located 0.2kb from other end.
If a DNA nucleotide sequence is CATAAAGCA, what is the mRNA nucleotide sequence that would base-pair with it?
Determine the cell biology of periodontal ligament tissues (PDL) originating from cranial neural-crest-derived extomesenchymal cells.
Discuss the events that take place with glucose levels and insulin secretion both after and meal and during exercise. Why might low blood sugar levels occur?
What is a fitness function? Describe how you can use a fitness function to: 1) determine whether an allele is dominant, co-dominant, or recessive AND beneficial, deleterious, neutral, or experiencing some other form of selection, and 2) predict th..
Discuss how would an increased number of Na+ leak channels in the membranes affect the sign and magnitude of the RMP? Justify your answer.
What parts of the autonomic nervous system control heart rate and what effect does each have on it. What parts of the heart contract as the wave passes them and in what order.
Which magnification was most helpful in making this determination? What optical principles are involved here and describe the role of each.
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